印度語口譯費用課程名稱︰分子生物學
課程性質︰必修
課程教師︰主授張麗冠（此範圍為張麗冠/林甫容老師授課）
開課學院：生命科學院
開課系所︰生化科技學系
考試日期（年月日）︰2017/01/09
考試時限（分鐘）：100分鐘
試題 :
(以下敘述均非與原題目完全相同，僅就考試主題作最接近的題目描述)
考試範圍：Ch 18~21 + Ch 15 (regulation部分)
Transcriptional Regulation in Prokaryotes
Transcriptional Regulation in Eukaryotes
Control of Translation
Regulatory RNAs
Gene Regulation in Development and Evolution
張老師部分：佔79% (Ch 18~20 + Ch 15 regulation部分)
1. Define the following terms. (30%)
(1) Dicer
(2) Heterochromatin Protein 1
(3) Aptamer
(4) Nonsense-mediated decay
(5) Internal Ribosome Entry Site
(6) Insulator
(7) Super Elongation Complex (SEC)
(8) Ribozyme switches
(9) RNase P
(10) Two component system and phosphorelay
2. Please describe the whole process of miRNA formation, as well as how it is
used to function in transcriptional gene silencing. Please also specify the
primary components it will use throughout formation. (8%)
3. How do you distinguish regulation in transcriptional initiation and
translational initiation? (8%)
※ Tip: 可用實驗設計說明
4. How are eIF2 and eIF4E involved in regulation of transcriptional initiation
in eukaryotes? (6%)
5. What is type II CRISPR-Cas immunity in bacteria? (8%)
(附上第七版課本兩張圖：
Figure 18-21: Map of bacteriophage λ in the circular form.
Figure 18-30: Genes and promoters involved in the lytic/lysogenic choice.)
6. How does a phage regulate itself to enter lysogenic phase?(6%) How does it
regulate the expression of integrase (Int) and excisionase (Xis)? (6%)
7. Read the following article and answer the questions.
The histone chaperone Spt6 coordinates histone H3K27 demethylation and
myogenesis.
EMBO J. 2013 Apr 17;32(8):1075-86.
Abstract
Histone chaperones affect chromatin structure and gene expression through
interaction with histones and RNA polymerase II (PolII). Here, we report
that the histone chaperone Spt6 counteracts H3K27me3, an epigenetic mark
deposited by the Polycomb Repressive Complex 2 (PRC2) and associated with
transcriptional repression. By regulating proper engagement and function of
the H3K27 demethylase KDM6A (UTX), Spt6 effectively promotes H3K27
demethylation, muscle gene expression, and cell differentiation. ChIP-Seq
experiments reveal an extensive genome-wide overlap of Spt6, PolII, and
KDM6A at transcribed regions that are devoid of H3K27me3. Mammalian cells
and zebrafish embryos with reduced Spt6 display increased H3K27me3 and
diminished expression of the master regulator MyoD, resulting in myogenic
differentiation defects. As a confirmation for an antagonistic relationship
between Spt6 and H3K27me3, inhibition of PRC2 permits MyoD re-expression in
myogenic cells with reduced Spt6. Our data indicate that, through
cooperation with PolII and KDM6A, Spt6 orchestrates removal of H3K27me3,
thus controlling developmental gene expression and cell differentiation.
(1) Define the function of CRP2. (3%)
(2) What is the function of H3K27me3? (3%)
(3) What is the function of Spt6? (3%)
-------------
林老師部分：佔25% (Ch 21)
1. Please explain the restricted expression of rhomboid in the ventral
neurogenic ectoderm. (5%)
2. Given the homozygous mutants below, please use "normal", "dorsalized" or
"ventralized" to describe what results you expect to see.
(1) Homozygous toll mutant (2%)
(2) Homozygous cactus mutant (2%)
3. What is the mechanism of localization of bicoid mRNA during oocyte?
(You can draw if drawing can help you) (5%)
4. Please describe the feature of Hunchback protein gradient due to the
distribution of Bicoid and Nanos that can explain for the proper embryo
formation. (8%)
5. You are screening the mRNA expression in the pair-rule gene even-skipped
(eve) with a homozygous mutant in Ubx gene that is loss of function.
Please describe the result you may expect to see. (3%)
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